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2.4 Photochemical derivatization is easy to operate, no need to use derivatization reagents (usually derivatization reagents are required after electrochemical column derivatization, the analysis cost is high, the operation is troublesome, and the instrument failure rate is high);
2.5 One-step purification, the recovery rate is as high as 90%.
3.2 Filtration: Filter the extracted sample quantitative filter paper and collect the filtrate;
2.3 HPLC+FLD+KRC post-column derivatization, effectively improve the detection sensitivity and achieve a lower detection limit (aflatoxin B1 0.05ppb).
2.4 Derivatization is convenient, and photochemical derivatization does not require the use of derivatization reagents (usually derivatization is required after electrochemical column derivatization, the analysis cost is high, the operation is troublesome, and the instrument failure rate is high).
3.2 Filtration: The extracted sample filter is passed through the glass fiber filter paper.
3.3 Install the regenerative immunoaffinity column on the pump flow rack for column purification: after the ribbofast aflatoxin total immunoaffinity column (IAC-010-3) activation, elution, elution three steps, elution The solution can be directly used for HPLC detection; 
â—HPLC-column: Aflatoxin-specific column 150 x 4.6 mm; C-18
â— Mobile phase: Methanol / water (45/ 55 v / v)
◠Flow rate: 1.0 mL/min Column temperature: 30 °C
◠Injection volume: 20μL
◠Fluorescence detector: λ-excitation wavelength: 365 nm λ- emission wavelength: 440 nm
Summary of aflatoxin analysis methods
Summary of aflatoxin analysis methods
1. Multi-functional purification column + KRC photochemical post-column derivatization method
1 Introduction
Multi-functional purification column is composed of a variety of solid phase composite fillers, including not only macromolecular substances, but more importantly, a variety of active lipophilic (non-polar) and charged (polar) substances, used to adsorb various esters, leaves Interferors such as flavin, chemicals, carbohydrates and proteins. Multi-functional purification column + KRC derivatization method is to use PRIBOLAB's Pribofast 226/260 multi-functional purification column to separate aflatoxins B1, B2, G1 and G2 well, and then use HPLC/FLD+KRC post-column derivatization system to be effective. The content of aflatoxin B1, B2, G1, G2 was determined.
2 Multi-functional purification column features:
2.1 Multi-functional purification column can simplify the sample extraction and purification steps without cleaning and elution.
2.2 Can achieve a variety of toxin detection, effectively reducing the cost of testing;
2.3 HPLC+FLD+KRC derivatization can effectively improve the detection sensitivity and achieve a lower detection limit (aflatoxin B1 0.05ppb);
2.6 Widely applicable to all kinds of agricultural products: grain, starch, animal feed, food, nuts, cottonseed, etc.
3 Multi-function purification column / KRC derivative method steps:
3.1 Extraction of samples: grinding and pulverizing, weighing 25g samples; mixing samples with acetonitrile/water (84/16 V/V),
High speed homogenization for 1 minute.
3.3 Take 4-6ml of filtrate quickly through the multi-functional purification column PribofastM226 or Pribofast M260
3.4 The column solution was collected and used directly for HPLC detection. The concentration of aflatoxin was directly recorded after KRC derivatization.
Second, immunoaffinity column + KRC photochemical post-column derivatization
1 Introduction
The Pribofast Aflatoxin Total Immunoaffinity Column selectively adsorbs toxins from the sample solution for very targeted purification of the toxin sample, which can then be used to efficiently determine aflatoxin B1 using the HPLC/FLD+KRC post-column derivatization system. , B2, G1, G2 concentration, improve the accuracy and sensitivity of detection.
2 Immunoaffinity column / KRC derivative characteristics:
2.1 The specificity is very strong, which makes the sample purification effect excellent;
2.2 Using the immunoaffinity column operating frame can make the sample clean quickly and improve the detection efficiency.
2.5 High recovery rate, reaching over 90%
3 Introduction of immunoaffinity column method:
3.1 Extraction of sample: grinding, weighing 50g sample; sample with methanol / water (80 / 20 V / V) mixture
Mix at high speed for 1 minute.
3.4 Determination: direct injection of HPLC, direct recording of aflatoxin concentration after column and KRC photochemical column derivatization;
Third, the total amount of aflatoxin detected by enzyme-linked immunosorbent assay
1 A direct competition ELISA method is used to determine the total amount of aflatoxin in the sample by measuring the absorbance of the sample and the sample of the standard using a microplate reader.
2 kit detection time: 15 minutes + 15 minutes
3 Detection limit: 0.1ppb
4 Cross-reaction rate:
Aflatoxin B1
100%
Aflatoxin B2
80%
Aflatoxin G1
101%
Aflatoxin G2
100%
AflatoxinM1
25%
About Pribofast KRC Post-column Derivatives and HPLC Parameters
â—Pribofast KRC post-column photochemical derivative wavelength: 254 nm
â—High performance liquid chromatograph needs to be prepared: PriboLab company Pribofast KRC post-column photochemical derivatizer, containing 1ml reaction cell, nano tube 254nm
Special column for aflatoxin:
product code
Product name and specifications
Quantity
IAC-010-3
Pribofast, Aflatoxins B1, B2, G1, G2 25 columns/pck 3mL, widebore column
Aflatoxin total immunoaffinity column 3ml, 25 / box
1 box
M226
PriboFast®226 Multifunctional cleanup column for Aflatoxin and zearalenone
Mycotoxin multi-purpose purification column for aflatoxin zearalenone detection 25 / box
1 box
PriboFast® 260
PriboFast® 260 Multifunctional cleanup column for Aflatoxin and zearalenone
Mycotoxin multi-purpose purification column for aflatoxin zearalenone detection 25 / box
1 box
PR K010-96
Pribofast ELISA Kit (96-well plate/stripes) for detection of Aflatoxin B1, B2, G1, G2 in food and feed samples
Aflatoxin total ELISA kit, 96 wells
1 box
STD#1081
Standard, Solution of 4 different aflatoxins in acetonitrile B1, G1
Aflatoxin B1B2G1G2 mixed standard. 1ml, 4 degrees dark storage
Four separate solids standards for aflatoxin B1B2G1G2 are also available.
1 box
KRC-25
Photochemical Reactor for Enhanced Detection Aflatoxin B1, B2, G1, G2; Pribofast KRC-25 230V 50 HzUVE 254 nm lamp
Pribofast KRC photochemical post-column derivatization system, 254nm nano UV lamp
1 set
ODS-150
HPLC-Column for Aflatoxin Analysis, C18 150 x 4.6 mm Aflatoxin-specific LC column
1
Pump Flow Operator / Immunoaffinity Column Operator for Immunoaffinity Column Sample Purification
(With gas pump adapter and other accessories, multi-purpose connector is optional)
1 set
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About PriboLab & Beijing Tellabs Technology Co., Ltd.
As one of the leading providers of solutions for mycotoxin detection technology worldwide, PriboLab provides professional mycotoxins for the global agricultural production, food processing and food and feed industries, with a strong research and development team and research on mycotoxin detection technology. Testing technology products and technical services. As the only partner of PriboLab in China, Beijing Tellabs Technology actively provides high-quality mycotoxin detection technology and product services to domestic customers, providing domestic customers with suitable mycotoxin detection solutions to help customers establish efficient and high-quality mycotoxin project testing. Analysis platform.